Comparative Study on the Predominance of Lactobacillus spp. and Escherichia Coli in Healthy vs Colibacillosis Diseased Broilers

This study aims to identify relative proportions of beneficial and pathogenic bacteria in the gut broilers risk factors that may be contributing development colibacillosis disease broiler farms District Kasur, Punjab, Pakistan. For this, 10 healthy affected were surveyed for ileum blood sample collection along with data regarding farm management, antibiotic use hygiene practices. Lactobacillus Escherichia coli number was estimated using Miles Misra method confirmed by Congo red dye assay. E. identified biochemically. analysis chi-square performed find any significant association between health status factors. Results showed during conditions counts differ significantly (p<0.05). (106-108 107-109) increased (p<0.05) about three folds decrease 105-107) four conditions. Risk factor associated non-vaccinated flocks, natural ventilation systems, rodent presence lack outfit disinfection or change workers when moving different houses. It is concluded work antagonistically each other. However, further research necessary determine exact mechanisms which influence colibacillosis. While as probiotic help prevention, good management practices are still crucial preventing spread disease.